V Gayathri
Jawaharlal Nehru Tropical Botanic Garden and Research Institute, India
Title: Chitosan asiaticoside nanoparticles synthesized from Centella asiatica for glioma therapy
Biography
Biography: V Gayathri
Abstract
Centella asiatica is a high value medicinal plant widely distributed in the tropical and sub-tropical regions. The main active principles are asiaticoside and madecassoside. Both these compounds have wide spectrum of biological activities. The plant is famous in the label of memory booster and it shows neuropharmacological activity. The brain targeted delivery of a drug is very challenging. Previous studies have shown that the penetrability of drug inside the brain is limited due to the presence of defensive mechanism of brain. Free diffusion transposition through the interstitium of the brain is restricted by complex anatomy of blood brain barrier (BBB). The poor solubility and low penetrability of a drug into the brain is the major drawback in the neuropharmacology related studies. To overcome these limitations nanovehicles were used. To enhance the permeability of a drug into the site of action was performed using nano delivery systems were developed. The brain permeability is a risk factor for a drug to exhibit therapeutic effects at a target site. The main objective of our study focuses on the development of a novel drug-based nanoparticle for the effective brain targeted delivery. Ionic gelation method was used for the encapsulation of asiaticoside into the chitosan alginate nanoparticle. Physicochemical and Biological characterization: The physical and chemical characterization study was done by Fourier transform infrared (FTIR), size distribution measurements using dynamic light scattering (DLS), surface morphology by scanning electron microscopy, internal structure by transmission electron microscopy and nature of the particle were analyzed by X-ray diffraction. The thermal characteristics were studied using thermo gravimetric analysis (TGA), differential scanning calorimetry (DSC) and differential thermal analysis (DTA). Interfacing the nanoparticles with glioma cells showed significant death of the cells. The work concludes that Chitosan asiaticoside nanoparticles are potential remedy for the second level treatment of glioma.