Universidad de Santiago de Compostela, Spain
Title: LIPSS stimulate stem mesenchymal cells differentiation to osteoblasts in titanium and tantalium
Biography: Alberto Jorge Mora
Orthopaedic surgery has grown from the hand of new materials that made possible to perform procedures as total hip replacement with feasibility. These procedures are common in most orthopaedic departments, and more than 70 000 hip or knee replacements are performed in Spain every year. The survival of these implants is critical to prevent loosening and the need for revision arthroplasty. The ideal surface to interact with bone has not been created. We created laser induced periodic surface structures (LIPSS) in the surface of titanium and tantalium to study the behaviour of stem cells compared to polished surfaces. We created 12 discs of each material and polished them. Later we created LIPSS in 6 discs of each material. We cultured them in human stem cells in a concentration of 25000 cell per cm2 for 20 days. We determined MTT, TNF-Alfa, alkaline phosphatase, IL-6, osteopontin and osteocalcin every 5 days until the day 20. We confirmed outcomes behave as a normal distribution after applying the Kolmogorov Smirnov test. We compared materials and surfaces with the T-student test. We accepted a difference of 0.05 as significant. LIPSS created increase statistically cell metabolism (best values in MTT assay) and decrease inflammatory response to the material (IL-6 and TNF-alfa values). Collagen is produced in more quantity and cells differentiate to osteoblast easily. These differences are seen from the beginning until the endpoint (day 20). LIPSS improved osteogenic properties of titanium and tantalium compared to smooth surfaces.